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Disordered cellular communication in TSCST. ( A ) Bar plot showing the number of inferred interactions in the leading edge and the tumor core. ( B ) Bar plot showing the interaction strength in the leading edge and the tumor core. ( C ) Circle plots display ligand-receptor interaction weights between distinct cellular components in the leading edge and the tumor core. Colors distinguish cell types. The circle size is proportional to the number of cells in each cluster, and the edge width represents communication probability. ( D ) Dot plots show the increased ligand-receptor interactions on the leading edge. ( E ) Dot plots showing the increased ligand-receptor interactions in the tumor core. ( F ) Dot plots show increased signaling <t>in</t> <t>macrophages</t> to tumor and Sertoli cells. ( G ) Feature plots of <t>APP-CD74</t> by ST in Patient 3 and Patient 4. ( H ) Feature plots of MDK-NCL by ST in Patient 3 and Patient 4. ( I ) Mapped SPP and MK signaling pathways in ST, colored by Seurat clusters. ( J ) Violin plots showing the expression level of APP-CD74 genes across the cell types in the leading edge and the tumor core, respectively. The y-axis shows the normalized read count. ( K ) Representative immunofluorescence images showing expression of CTNNB1 and CD74 (n = 4). Nuclei were stained with DAPI. ( L ) Relative mRNA expression of MRC1 and ARG1 (M2-type macrophage markers) and IL1B (M1-type macrophage marker) in macrophages treated with APP. ( M ) Relative mRNA expression of MMP2 (a marker associated with phagocytic function in macrophages) in macrophages treated with APP. The P value was calculated by Welch’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001
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Disordered cellular communication in TSCST. ( A ) Bar plot showing the number of inferred interactions in the leading edge and the tumor core. ( B ) Bar plot showing the interaction strength in the leading edge and the tumor core. ( C ) Circle plots display ligand-receptor interaction weights between distinct cellular components in the leading edge and the tumor core. Colors distinguish cell types. The circle size is proportional to the number of cells in each cluster, and the edge width represents communication probability. ( D ) Dot plots show the increased ligand-receptor interactions on the leading edge. ( E ) Dot plots showing the increased ligand-receptor interactions in the tumor core. ( F ) Dot plots show increased signaling <t>in</t> <t>macrophages</t> to tumor and Sertoli cells. ( G ) Feature plots of <t>APP-CD74</t> by ST in Patient 3 and Patient 4. ( H ) Feature plots of MDK-NCL by ST in Patient 3 and Patient 4. ( I ) Mapped SPP and MK signaling pathways in ST, colored by Seurat clusters. ( J ) Violin plots showing the expression level of APP-CD74 genes across the cell types in the leading edge and the tumor core, respectively. The y-axis shows the normalized read count. ( K ) Representative immunofluorescence images showing expression of CTNNB1 and CD74 (n = 4). Nuclei were stained with DAPI. ( L ) Relative mRNA expression of MRC1 and ARG1 (M2-type macrophage markers) and IL1B (M1-type macrophage marker) in macrophages treated with APP. ( M ) Relative mRNA expression of MMP2 (a marker associated with phagocytic function in macrophages) in macrophages treated with APP. The P value was calculated by Welch’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001
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Disordered cellular communication in TSCST. ( A ) Bar plot showing the number of inferred interactions in the leading edge and the tumor core. ( B ) Bar plot showing the interaction strength in the leading edge and the tumor core. ( C ) Circle plots display ligand-receptor interaction weights between distinct cellular components in the leading edge and the tumor core. Colors distinguish cell types. The circle size is proportional to the number of cells in each cluster, and the edge width represents communication probability. ( D ) Dot plots show the increased ligand-receptor interactions on the leading edge. ( E ) Dot plots showing the increased ligand-receptor interactions in the tumor core. ( F ) Dot plots show increased signaling <t>in</t> <t>macrophages</t> to tumor and Sertoli cells. ( G ) Feature plots of <t>APP-CD74</t> by ST in Patient 3 and Patient 4. ( H ) Feature plots of MDK-NCL by ST in Patient 3 and Patient 4. ( I ) Mapped SPP and MK signaling pathways in ST, colored by Seurat clusters. ( J ) Violin plots showing the expression level of APP-CD74 genes across the cell types in the leading edge and the tumor core, respectively. The y-axis shows the normalized read count. ( K ) Representative immunofluorescence images showing expression of CTNNB1 and CD74 (n = 4). Nuclei were stained with DAPI. ( L ) Relative mRNA expression of MRC1 and ARG1 (M2-type macrophage markers) and IL1B (M1-type macrophage marker) in macrophages treated with APP. ( M ) Relative mRNA expression of MMP2 (a marker associated with phagocytic function in macrophages) in macrophages treated with APP. The P value was calculated by Welch’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001
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Disordered cellular communication in TSCST. ( A ) Bar plot showing the number of inferred interactions in the leading edge and the tumor core. ( B ) Bar plot showing the interaction strength in the leading edge and the tumor core. ( C ) Circle plots display ligand-receptor interaction weights between distinct cellular components in the leading edge and the tumor core. Colors distinguish cell types. The circle size is proportional to the number of cells in each cluster, and the edge width represents communication probability. ( D ) Dot plots show the increased ligand-receptor interactions on the leading edge. ( E ) Dot plots showing the increased ligand-receptor interactions in the tumor core. ( F ) Dot plots show increased signaling <t>in</t> <t>macrophages</t> to tumor and Sertoli cells. ( G ) Feature plots of <t>APP-CD74</t> by ST in Patient 3 and Patient 4. ( H ) Feature plots of MDK-NCL by ST in Patient 3 and Patient 4. ( I ) Mapped SPP and MK signaling pathways in ST, colored by Seurat clusters. ( J ) Violin plots showing the expression level of APP-CD74 genes across the cell types in the leading edge and the tumor core, respectively. The y-axis shows the normalized read count. ( K ) Representative immunofluorescence images showing expression of CTNNB1 and CD74 (n = 4). Nuclei were stained with DAPI. ( L ) Relative mRNA expression of MRC1 and ARG1 (M2-type macrophage markers) and IL1B (M1-type macrophage marker) in macrophages treated with APP. ( M ) Relative mRNA expression of MMP2 (a marker associated with phagocytic function in macrophages) in macrophages treated with APP. The P value was calculated by Welch’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001
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Disordered cellular communication in TSCST. ( A ) Bar plot showing the number of inferred interactions in the leading edge and the tumor core. ( B ) Bar plot showing the interaction strength in the leading edge and the tumor core. ( C ) Circle plots display ligand-receptor interaction weights between distinct cellular components in the leading edge and the tumor core. Colors distinguish cell types. The circle size is proportional to the number of cells in each cluster, and the edge width represents communication probability. ( D ) Dot plots show the increased ligand-receptor interactions on the leading edge. ( E ) Dot plots showing the increased ligand-receptor interactions in the tumor core. ( F ) Dot plots show increased signaling <t>in</t> <t>macrophages</t> to tumor and Sertoli cells. ( G ) Feature plots of <t>APP-CD74</t> by ST in Patient 3 and Patient 4. ( H ) Feature plots of MDK-NCL by ST in Patient 3 and Patient 4. ( I ) Mapped SPP and MK signaling pathways in ST, colored by Seurat clusters. ( J ) Violin plots showing the expression level of APP-CD74 genes across the cell types in the leading edge and the tumor core, respectively. The y-axis shows the normalized read count. ( K ) Representative immunofluorescence images showing expression of CTNNB1 and CD74 (n = 4). Nuclei were stained with DAPI. ( L ) Relative mRNA expression of MRC1 and ARG1 (M2-type macrophage markers) and IL1B (M1-type macrophage marker) in macrophages treated with APP. ( M ) Relative mRNA expression of MMP2 (a marker associated with phagocytic function in macrophages) in macrophages treated with APP. The P value was calculated by Welch’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001
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Disordered cellular communication in TSCST. ( A ) Bar plot showing the number of inferred interactions in the leading edge and the tumor core. ( B ) Bar plot showing the interaction strength in the leading edge and the tumor core. ( C ) Circle plots display ligand-receptor interaction weights between distinct cellular components in the leading edge and the tumor core. Colors distinguish cell types. The circle size is proportional to the number of cells in each cluster, and the edge width represents communication probability. ( D ) Dot plots show the increased ligand-receptor interactions on the leading edge. ( E ) Dot plots showing the increased ligand-receptor interactions in the tumor core. ( F ) Dot plots show increased signaling <t>in</t> <t>macrophages</t> to tumor and Sertoli cells. ( G ) Feature plots of <t>APP-CD74</t> by ST in Patient 3 and Patient 4. ( H ) Feature plots of MDK-NCL by ST in Patient 3 and Patient 4. ( I ) Mapped SPP and MK signaling pathways in ST, colored by Seurat clusters. ( J ) Violin plots showing the expression level of APP-CD74 genes across the cell types in the leading edge and the tumor core, respectively. The y-axis shows the normalized read count. ( K ) Representative immunofluorescence images showing expression of CTNNB1 and CD74 (n = 4). Nuclei were stained with DAPI. ( L ) Relative mRNA expression of MRC1 and ARG1 (M2-type macrophage markers) and IL1B (M1-type macrophage marker) in macrophages treated with APP. ( M ) Relative mRNA expression of MMP2 (a marker associated with phagocytic function in macrophages) in macrophages treated with APP. The P value was calculated by Welch’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001
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Disordered cellular communication in TSCST. ( A ) Bar plot showing the number of inferred interactions in the leading edge and the tumor core. ( B ) Bar plot showing the interaction strength in the leading edge and the tumor core. ( C ) Circle plots display ligand-receptor interaction weights between distinct cellular components in the leading edge and the tumor core. Colors distinguish cell types. The circle size is proportional to the number of cells in each cluster, and the edge width represents communication probability. ( D ) Dot plots show the increased ligand-receptor interactions on the leading edge. ( E ) Dot plots showing the increased ligand-receptor interactions in the tumor core. ( F ) Dot plots show increased signaling <t>in</t> <t>macrophages</t> to tumor and Sertoli cells. ( G ) Feature plots of <t>APP-CD74</t> by ST in Patient 3 and Patient 4. ( H ) Feature plots of MDK-NCL by ST in Patient 3 and Patient 4. ( I ) Mapped SPP and MK signaling pathways in ST, colored by Seurat clusters. ( J ) Violin plots showing the expression level of APP-CD74 genes across the cell types in the leading edge and the tumor core, respectively. The y-axis shows the normalized read count. ( K ) Representative immunofluorescence images showing expression of CTNNB1 and CD74 (n = 4). Nuclei were stained with DAPI. ( L ) Relative mRNA expression of MRC1 and ARG1 (M2-type macrophage markers) and IL1B (M1-type macrophage marker) in macrophages treated with APP. ( M ) Relative mRNA expression of MMP2 (a marker associated with phagocytic function in macrophages) in macrophages treated with APP. The P value was calculated by Welch’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001
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Image Search Results


Disordered cellular communication in TSCST. ( A ) Bar plot showing the number of inferred interactions in the leading edge and the tumor core. ( B ) Bar plot showing the interaction strength in the leading edge and the tumor core. ( C ) Circle plots display ligand-receptor interaction weights between distinct cellular components in the leading edge and the tumor core. Colors distinguish cell types. The circle size is proportional to the number of cells in each cluster, and the edge width represents communication probability. ( D ) Dot plots show the increased ligand-receptor interactions on the leading edge. ( E ) Dot plots showing the increased ligand-receptor interactions in the tumor core. ( F ) Dot plots show increased signaling in macrophages to tumor and Sertoli cells. ( G ) Feature plots of APP-CD74 by ST in Patient 3 and Patient 4. ( H ) Feature plots of MDK-NCL by ST in Patient 3 and Patient 4. ( I ) Mapped SPP and MK signaling pathways in ST, colored by Seurat clusters. ( J ) Violin plots showing the expression level of APP-CD74 genes across the cell types in the leading edge and the tumor core, respectively. The y-axis shows the normalized read count. ( K ) Representative immunofluorescence images showing expression of CTNNB1 and CD74 (n = 4). Nuclei were stained with DAPI. ( L ) Relative mRNA expression of MRC1 and ARG1 (M2-type macrophage markers) and IL1B (M1-type macrophage marker) in macrophages treated with APP. ( M ) Relative mRNA expression of MMP2 (a marker associated with phagocytic function in macrophages) in macrophages treated with APP. The P value was calculated by Welch’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001

Journal: Biomarker Research

Article Title: Single-cell and spatial transcriptome analysis reveals the potential therapeutic targets for testicular sex cord-stromal cell tumor

doi: 10.1186/s40364-026-00924-0

Figure Lengend Snippet: Disordered cellular communication in TSCST. ( A ) Bar plot showing the number of inferred interactions in the leading edge and the tumor core. ( B ) Bar plot showing the interaction strength in the leading edge and the tumor core. ( C ) Circle plots display ligand-receptor interaction weights between distinct cellular components in the leading edge and the tumor core. Colors distinguish cell types. The circle size is proportional to the number of cells in each cluster, and the edge width represents communication probability. ( D ) Dot plots show the increased ligand-receptor interactions on the leading edge. ( E ) Dot plots showing the increased ligand-receptor interactions in the tumor core. ( F ) Dot plots show increased signaling in macrophages to tumor and Sertoli cells. ( G ) Feature plots of APP-CD74 by ST in Patient 3 and Patient 4. ( H ) Feature plots of MDK-NCL by ST in Patient 3 and Patient 4. ( I ) Mapped SPP and MK signaling pathways in ST, colored by Seurat clusters. ( J ) Violin plots showing the expression level of APP-CD74 genes across the cell types in the leading edge and the tumor core, respectively. The y-axis shows the normalized read count. ( K ) Representative immunofluorescence images showing expression of CTNNB1 and CD74 (n = 4). Nuclei were stained with DAPI. ( L ) Relative mRNA expression of MRC1 and ARG1 (M2-type macrophage markers) and IL1B (M1-type macrophage marker) in macrophages treated with APP. ( M ) Relative mRNA expression of MMP2 (a marker associated with phagocytic function in macrophages) in macrophages treated with APP. The P value was calculated by Welch’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001

Article Snippet: THP-1-induced macrophages treated with APP protein (MedChemExpress, Shanghai, Catalogue number: HY- P72834 ) were collected, and total RNA was extracted using TRIzol Reagent (Invitrogen, 15596018CN, USA).

Techniques: Protein-Protein interactions, Expressing, Immunofluorescence, Staining, Marker